H-Phe(4-N3)

References

H-Phe(4-N3) is used in protein biosynthesis as unnatural amino acid for genetic code expansion. The azido-funcionality can be further utilized to cross-link various functionalities to proteins utilizing click-chemistry.

https://doi.org/10.1126/science.1084772

https://doi.org/10.1038/nature08817

https://doi.org/10.1038/nature14095

https://doi.org/10.1021/acscentsci.1c00499

The azido-functionality is IR-active and can be used in protein active site electrostatic environment studies.

https://doi.org/10.1038/nchembio.167

Angew. Chem. Int. Ed. 2022, 61, e202200648;

https://doi.org/10.1002/anie.202200648

The azide-handle can also be used for protein PEGylation or attachment to antibodies using click-chemistry.

https://doi.org/10.1021/acssynbio.7b00316